in your G-code toolpath, you set G90 (absolute distance mode) and lift Z to its machine zero (G53 G0 Z0). Units are set to inches. Then subsequently you have a command G0 Z3.2. This is not possible. If you are already on the topmost Z position which is Z=0, you cannot move further upwards. The Z travel on Z-16 axis goes from 0 to −5.25 in (0 to −133 mm). In machine coordinates, Z axis has only negative values, because Z zero is at topmost position and in the right-hand cartesian coordinate system which RS274/NGC machines use, Z values increase towards top and decrease towards bottom, so can only be negative if topmost is zero.
By the way, you can also upload g-code files (.ngc) in a posting. You get the next level of membership if you read a number of posts. Then you can post more things.