I’ll give it one more try. I think I have ISP programmer on my stash. What else I can do anyway while waiting for my SLB.
I will also check the connectivity of the DB25 pins to the PCB. You might be right cause the cable I use from my prototype AutoDustBoot was tangled during the x-gantry move because I haven’t put it inside drag chain. So might be likely that it pull some solder pins. But not sure about LOAD-2, even the LOAD-2 port on the back on the controller is not triggering the Vcc.